Evaluation of immunohistochemical expression of collagen type I in bone defect treated by local application of collagen and Beta – Tricalcium phosphate in rats
1
Department of Oral Diagnosis, College of Dentistry, University of Baghdad, Iraq
Submission date: 2024-01-07
Final revision date: 2024-08-20
Acceptance date: 2024-09-02
Publication date: 2024-11-03
Wiadomości Lekarskie 2024;77(9):1686-1692
KEYWORDS
TOPICS
ABSTRACT
Aim:
Background: As a part of the healing process after an injury, during skeletal development, or during ongoing remodeling throughout adulthood, bone has the innate ability to regenerate. Aims: This study aimed to estimate outcome of combined application of collagen/β- tricalcium phosphate (Coll /β- TCP) on bone defect healing by immunohistochemical localization of collagen type I.
Material and methods:
Material and Methods: A total of 20 adult male rats (albino rats) were used in this study. Four intra bony defects were created in both femurs of each rat, three defects were treated with Coll, β- TCP and combination of Coll /β- TCP while forth defect left to heal spontaneously as control. Scarification of animals was done after 2- and 4-weeks healing periods (10 rats for each). Immunohistochemical localization of collagen type I (COL1) monoclonal antibody was done for all bone specimens.
Results:
Results: Immunohistochemical results showed positive expression of (COL1) by bone cells osteoblast (OB), osteocyte (OC), osteoclast (OCL) and bone marrow stromal cells (BMSCs) at 2- and 4-week duration for all examined groups with variable rates of intensity. Mean values of immunoreactive score (IRS) decreased with time for bone cells and for BMSCs except for OC where values increased with time as the highest values were recorded in combination group at 4 weeks.
Conclusions:
Conclusion: Combined application of (Coll /β- TCP) was effective in accelerating bone healing process through elevating the IRS of (COL1) noticed by osteoblast, osteocyte and bone marrow stromal cells
Background: As a part of the healing process after an injury, during skeletal development, or during ongoing remodeling throughout adulthood, bone has the innate ability to regenerate. Aims: This study aimed to estimate outcome of combined application of collagen/β- tricalcium phosphate (Coll /β- TCP) on bone defect healing by immunohistochemical localization of collagen type I.
Material and methods:
Material and Methods: A total of 20 adult male rats (albino rats) were used in this study. Four intra bony defects were created in both femurs of each rat, three defects were treated with Coll, β- TCP and combination of Coll /β- TCP while forth defect left to heal spontaneously as control. Scarification of animals was done after 2- and 4-weeks healing periods (10 rats for each). Immunohistochemical localization of collagen type I (COL1) monoclonal antibody was done for all bone specimens.
Results:
Results: Immunohistochemical results showed positive expression of (COL1) by bone cells osteoblast (OB), osteocyte (OC), osteoclast (OCL) and bone marrow stromal cells (BMSCs) at 2- and 4-week duration for all examined groups with variable rates of intensity. Mean values of immunoreactive score (IRS) decreased with time for bone cells and for BMSCs except for OC where values increased with time as the highest values were recorded in combination group at 4 weeks.
Conclusions:
Conclusion: Combined application of (Coll /β- TCP) was effective in accelerating bone healing process through elevating the IRS of (COL1) noticed by osteoblast, osteocyte and bone marrow stromal cells
| eISSN: | 2719-342X |
| ISSN: | 0043-5147 |
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