Histopathological, Immunohistochemical and Physiological study for the Hepatoprotective effect of melatonin against Inhrifampicin induced hepatotoxicity in mice model
1
Department of Human Anatomy, Faculty of Medicine, University of Kufa, Najaf, Iraq, Iraq
2
Al-Abraar Secondary School,, Ministry of Education, Baghdad, Iraq, Iraq
3
Department of Pathology and Forensic Medicine, Faculty of Medicine, University of Kufa, Najaf, Iraq, Iraq
4
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa, Najaf, Iraq, Iraq
Submission date: 2024-06-07
Final revision date: 2024-07-16
Acceptance date: 2024-08-12
Publication date: 2024-11-03
Corresponding author
Rasha Hatem Dosh
Department of Human Anatomy, Faculty of Medicine, University of Kufa, Najaf, Iraq, Iraq
Department of Human Anatomy, Faculty of Medicine, University of Kufa, Najaf, Iraq, Iraq
Wiadomości Lekarskie 2024;77(9):1745-1752
KEYWORDS
TOPICS
ABSTRACT
Aim:
Aim: The purpose of this study is to assess the hepatoprotective effect of melatonin against isoniazid (INH) and rifampicin (RMP) induced hepatotoxicity in albino mice.
Material and methods:
Materials and Methods: Adult male mice were divided into four groups: saline, INH-RMP, INH-RMP+MT and MT were administered for 21 days. Biochemical analyses were performed for the determination of ALT, AST. Histopathological changes in the liver and Immunohistochemical assessment to determine the expression of Caspace3 were also examined.
Results:
Results: Biochemical analysis revealed significant increases in serum ALT and AST in INH-RMP group. Histopathological findings demonstrated severe liver damage in INH-RMP group as compared with control group. In contrast, treatment of mice with melatonin (MT) markedly mitigated the liver injury. Immunohistochemical findings demonstrated apoptotic marker caspace3 significantly higher in INH-RMP group as compared with control group.
Conclusions:
Conclusions: Experimental findings highlight the potential benefits of melatonin in this model, prompting speculation on its potential application in human therapy.
Aim: The purpose of this study is to assess the hepatoprotective effect of melatonin against isoniazid (INH) and rifampicin (RMP) induced hepatotoxicity in albino mice.
Material and methods:
Materials and Methods: Adult male mice were divided into four groups: saline, INH-RMP, INH-RMP+MT and MT were administered for 21 days. Biochemical analyses were performed for the determination of ALT, AST. Histopathological changes in the liver and Immunohistochemical assessment to determine the expression of Caspace3 were also examined.
Results:
Results: Biochemical analysis revealed significant increases in serum ALT and AST in INH-RMP group. Histopathological findings demonstrated severe liver damage in INH-RMP group as compared with control group. In contrast, treatment of mice with melatonin (MT) markedly mitigated the liver injury. Immunohistochemical findings demonstrated apoptotic marker caspace3 significantly higher in INH-RMP group as compared with control group.
Conclusions:
Conclusions: Experimental findings highlight the potential benefits of melatonin in this model, prompting speculation on its potential application in human therapy.
| eISSN: | 2719-342X |
| ISSN: | 0043-5147 |
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