Aim:
Background: This study uses molecular docking to describes the potential binding affinity of new sulfonamides derivatives by incorporating substituted Isatin moiety as tail groups linked to 4-amino benzene sulfonamide as zinc binding group through a 4-amino ethyl benzoate linker, that targeted carbonic anhydrase XII enzyme in some firm tumors like breast and colon cancer by using CA XII (PDB: 1JCZ /chain A). Aims: to evaluate compound I, II, III, and IV's anticancer properties that have just been produced. These substances were created with the specific purpose of targeting solid tumors' carbonic anhydrase enzyme.

Material and methods:
Materials and method: The chemical synthesis involved the use of 4-aminobenzenesulfonamide, Ethyl 4-aminobenzoate, isatin and its derivatives, absolute ethanol, DMF, glacial acetic acid. Docking studies were conducted using the MOE software program version 2015.10.

Results:
Results: Since acetazolamide and the sulfanilamide group shared the same pharmacophore, they were chosen. When compared to acetazolamide, compounds II and III produced a maximum score and an irreversible relationship.

Conclusions:
Conclusion: Using the Molecular Operating Environment (MOE) software, the binding model and two values the RMSD and S.score—are computed for newly synthesized compounds. When compared to acetazolamide, the theoretically generated compounds showed promise results with these proteins and good binding affinities with the receptor active pocket (S. score: - 6.89, -7.12, -6.75).