Aim:
Aims: to investigate and compared the frequency of biofilm formation (ica A) and adhesion genes (clf A, cnaA, fnbA and fnbB) in chromosome and plasmid of ''S. saprophyticus".

Material and methods:
Materials and methods: 48 isolates about "S. saprophyticus" were selective randomly from total 1019 isolated from pregnant women with asymptomatic bacteriuria where the samples were collected in AL Habboby Hospital and Al-Hussein teaching, and bent Al-Huda hospitals from the first of April 2022 until the first of September 2023 and bacteria of study were identified depend on previous method.

Results:
Results: PCR appeared, higher existence percentage of study virulence factors genes was in chromosome that reached 399.9% of study bacteria, more than in its in plasmid 66.5%, frequency rate of study genes in chromosome was as: ica A and fnb A genes were complete, 100% (48/48), while cna A and Clf A genes percentage were identical and came in second grade that arrived significant rate 83.3% (40/48), the lowest results were showed 33.3% (16/48) isolates harbored fnbB gene, while they were decreased in itś plasmid as following : ica A and fnb A genes were 12.5% (6/48) and 22.9% (11/48)respectively, the cna A and Clf A genes percentage 6.2 % (3/48) and 4.1% (2/48) respectively, fnb B gene 20.8% (10/48).

Conclusions:
Conclusion: Depend on above data may be ica A, fnb A, clf A, cna A genes more occurrence in comparison to fnb B genes among "S. saprophyticus" and plasmid may double the rate of same virulence study genes.