Potential Cardioprotective effect of Trimetazidine in Mice model of endotoxemia: role of AMPK-Nrf2
1
Pharmacology and Therapeutic Department, Faculty of Medicine, University of Kufa, Iraq
Submission date: 2023-11-01
Final revision date: 2024-05-18
Acceptance date: 2024-07-04
Publication date: 2025-01-31
Corresponding author
Najah Rayish Hadi
Pharmacology and Therapeutic Department, Faculty of Medicine, University of Kufa, Iraq
Pharmacology and Therapeutic Department, Faculty of Medicine, University of Kufa, Iraq
Wiadomości Lekarskie 2025;(1):35-44
KEYWORDS
TOPICS
ABSTRACT
Aim:
Background: Polymicrobial sepsis is a life-threatening situation characterized by multiorgan dysfunction resulting from the body's abnormal response to microbial invasion. Aims: to clarify the potential cardioprotective effect of Trimetazidine against experimentally sepsis-caused endotoxic cardiac injury damage in mice.
Material and methods:
Material and method: 24 Mice were divided into four groups (n=6): Sham group, CLP group DMSO group, trimetazidine-treated group 50 mg/kg IP, 1hr before CLP, then the animals were sacrificed 24 hr after CLP and tissue sample was taken for measurement of TNF-Α, TNF-Αr1, IL-1β, HO-1, MPO, caspase-11, F2-isoprostane and serum troponin by ELISA and gene expression of AMPK-Nrf2 by qpcr and histopathological study.
Results:
Results: trimetazidine treated group showed significant changes as compared with clp group regarding TNF-α, TNF-αr1, IL-1β, HO-1, MPO, CASPASE-11, F2-ISOPROSTANE as well as affect tissue mRNA expression of AMPK-Nrf22 genes p<0.05.
Conclusions:
Conclusion: We evaluate that Trimetazidine has cardio protective effects due to its anti-inflammatory and anti-oxidative action. Also, trimetazidine showed a cardio-protective effect as they affect tissue mRNA expression of AMPK-Nrf2 genes.
Background: Polymicrobial sepsis is a life-threatening situation characterized by multiorgan dysfunction resulting from the body's abnormal response to microbial invasion. Aims: to clarify the potential cardioprotective effect of Trimetazidine against experimentally sepsis-caused endotoxic cardiac injury damage in mice.
Material and methods:
Material and method: 24 Mice were divided into four groups (n=6): Sham group, CLP group DMSO group, trimetazidine-treated group 50 mg/kg IP, 1hr before CLP, then the animals were sacrificed 24 hr after CLP and tissue sample was taken for measurement of TNF-Α, TNF-Αr1, IL-1β, HO-1, MPO, caspase-11, F2-isoprostane and serum troponin by ELISA and gene expression of AMPK-Nrf2 by qpcr and histopathological study.
Results:
Results: trimetazidine treated group showed significant changes as compared with clp group regarding TNF-α, TNF-αr1, IL-1β, HO-1, MPO, CASPASE-11, F2-ISOPROSTANE as well as affect tissue mRNA expression of AMPK-Nrf22 genes p<0.05.
Conclusions:
Conclusion: We evaluate that Trimetazidine has cardio protective effects due to its anti-inflammatory and anti-oxidative action. Also, trimetazidine showed a cardio-protective effect as they affect tissue mRNA expression of AMPK-Nrf2 genes.
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| eISSN: | 2719-342X |
| ISSN: | 0043-5147 |
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